biotin conjugated anti cd5l (R&D Systems)

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R&D Systems biotin conjugated anti cd5l
Biotin Conjugated Anti Cd5l, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+conjugated+anti+cd5l/CD5L+Antibody+%5BBiotin%5D/pmc07933155-315-18-20
Average 94 stars, based on 6 article reviews

biotin conjugated anti cd5l - by Bioz Stars, 2026-10

94/100 stars

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Sandwich ELISA:

Article Title: IL-23p19 and CD5 antigen-like form a possible novel heterodimeric cytokine and contribute to experimental autoimmune encephalomyelitis development
Article Snippet: The amount of CD5L was determined by ELISA according to the manufacturers’ instructions (Sino Biological). .. The amount of p19/CD5L was determined by standard sandwich ELISA protocol using anti-p19 (5B2) as a capture antibody, biotin-conjugated anti-CD5L (R&D Systems) as a detection antibody, and the purified recombinant hyper-p19/CD5L as a standard protein. ..

Purification:

Article Title: IL-23p19 and CD5 antigen-like form a possible novel heterodimeric cytokine and contribute to experimental autoimmune encephalomyelitis development
Article Snippet: The amount of CD5L was determined by ELISA according to the manufacturers’ instructions (Sino Biological). .. The amount of p19/CD5L was determined by standard sandwich ELISA protocol using anti-p19 (5B2) as a capture antibody, biotin-conjugated anti-CD5L (R&D Systems) as a detection antibody, and the purified recombinant hyper-p19/CD5L as a standard protein. ..

Recombinant:

Article Title: IL-23p19 and CD5 antigen-like form a possible novel heterodimeric cytokine and contribute to experimental autoimmune encephalomyelitis development
Article Snippet: The amount of CD5L was determined by ELISA according to the manufacturers’ instructions (Sino Biological). .. The amount of p19/CD5L was determined by standard sandwich ELISA protocol using anti-p19 (5B2) as a capture antibody, biotin-conjugated anti-CD5L (R&D Systems) as a detection antibody, and the purified recombinant hyper-p19/CD5L as a standard protein. ..

Staining:

Article Title: IL-23p19 and CD5 antigen-like form a possible novel heterodimeric cytokine and contribute to experimental autoimmune encephalomyelitis development
Article Snippet: These cells were then stained intracellularly with FITC- or PerCP-Cy5.5-conjugated anti-IFN-γ (XMG1.2, BioLegend, eBioscience), APC-conjugated anti-IL-10 (JES5-16E3, BioLegend), PE-Cy7-conjugated anti-IL-17A (TC11-18H10, BioLegend), and PE-conjugated anti-GM-CSF (MP1-22E9, BioLegend). .. For intracellular staining of CD5L and p19, cells were stained with PE-conjugated anti-CD4 (GK1.5, BioLegend), fixed, permeabilized, and then stained intracellularly with biotin-conjugated anti-CD5L (R&D Systems), and eFluor 660-conjugated anti-p19 (fc23cpg, eBioscience), followed by Brilliant Violet 421-cojugated streptavidin (BioLegend). .. For intracellular staining of pY-STAT5, naive CD4 + T cells were stimulated with plate-bound anti-CD3 and anti-CD28 under Th conditions for 3 days, washed with PBS, and rested in 10% FBS medium for 6 h. These cells were then restimulated with recombinant p19, CD5L, p19/CD5L, and IL-2 (100 U/ml) for 20 min, and then fixed, permeabilized with ice-cold 90% methanol, and then stained intracellularly with Alexa Fluor 647-conjugated anti-pY-STAT5 (Tyr694) (C71E5, Cell Signaling) or Alexa Fluor 647-conjugated rabbit mAb IgG XP isotype control (DA1E, Cell Signaling) according to the manufacturers’ instructions.

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